Green- to far-red-emitting fluorogenic tetrazine probes – synthetic access and no-wash protein imaging inside living cells† †Electronic supplementary information (ESI) available: Synthetic procedures and spectroscopic data, details of cell experiments and imaging. See DOI: 10.1039/c6sc03879d Click here for additional data file.

Green- to far-red-emitting fluorogenic tetrazine probes - synthetic access and no-wash protein imaging inside living cells.

Fluorogenic probes for bioorthogonal labeling chemistry are highly beneficial to reduce background signal in fluorescence microscopy imaging. 1,2,4,5-Tetrazines are known substrates for the bioorthogonal inverse electron demand Diels-Alder reaction (DAinv) and tetrazine substituted fluorophores can exhibit fluorogenic properties. Herein, we report the synthesis of a palette of novel fluorogenic...

A far-red emitting probe for unambiguous detection of mobile zinc in acidic vesicles and deep tissue† †Electronic supplementary information (ESI) available: General methods, synthetic procedures, NMR spectra, kinetic data, pH profile, metal selectivity graph, cell culture and staining protocols, wide-field microscopy experiments, tissue specimen preparation, and two-photon imaging techniques. See DOI: 10.1039/c4sc03388d Click here for additional data file.

Imaging mobile zinc in acidic environments remains challenging because most small-molecule optical probes display pH-dependent fluorescence. Here we report a reaction-based sensor that detects mobile zinc unambiguously at low pH. The sensor responds reversibly and with a large dynamic range to exogenously applied Zn2+ in lysosomes of HeLa cells, endogenous Zn2+ in insulin granules of MIN6 cells...

π-extended anthracenes as sensitive probes for mechanical stress† †Electronic supplementary information (ESI) available: Detailed synthetic, spectroscopic, kinetic and mechanochemical procedures. See DOI: 10.1039/c5sc03297k Click here for additional data file.

Self-assembling α,γ-cyclic peptides that generate cavities with tunable properties† †Electronic supplementary information (ESI) available: Synthetic experimental details, DFT calculation details, additional figures (relevant NMR data) and schemes (synthetic schemes for peptide and amino acid preparation). CCDC 1400134. For ESI and crystallographic data in CIF or other electronic format see DOI: 10.1039/c5sc03187g Click here for additional data file. Click here for additional data file.

Engineering a FRET strategy to achieve a ratiometric two-photon fluorescence response with a large emission shift and its application to fluorescence imaging† †Electronic supplementary information (ESI) available: Synthetic procedures and characterization data; additional spectroscopic data. See DOI: 10.1039/c4sc03883e Click here for additional data file.